RNA

Characterizing the replicability of cell types defined by single cell RNA-sequencing data using MetaNeighbor

Single-cell RNA-sequencing (scRNA-seq) technology provides a new avenue to discover and characterize cell types; however, the experiment-specific technical biases and analytic variability inherent to current pipelines may undermine its replicability. …

Characterizing the replicability of cell types defined by single cell RNA-sequencing data using MetaNeighbor

Single-cell RNA-sequencing (scRNA-seq) technology provides a new avenue to discover and characterize cell types; however, the experiment-specific technical biases and analytic variability inherent to current pipelines may undermine its replicability. …

Exploiting single-cell expression to characterize co-expression replicability

BACKGROUND: Co-expression networks have been a useful tool for functional genomics, providing important clues about the cellular and biochemical mechanisms that are active in normal and disease processes. However, co-expression analysis is often …

AuPairWise: A Method to Estimate RNA-Seq Replicability through Co-expression

In addition to detecting novel transcripts and higher dynamic range, a principal claim for RNA-sequencing has been greater replicability, typically measured in sample-sample correlations of gene expression levels. Through a re-analysis of ENCODE …

Guidance for RNA-seq co-expression network construction and analysis: safety in numbers

MOTIVATION: RNA-seq co-expression analysis is in its infancy and reasonable practices remain poorly defined. We assessed a variety of RNA-seq expression data to determine factors affecting functional connectivity and topology in co-expression …